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ddhCTP

Catalog No.
B8293
Chain terminator for the RNA-dependent RNA polymerases
Grouped product items
SizePriceStock Qty
5mg
$1,155.00
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25mg
$3,465.00
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Background

Viperin, a member of the radical S-adenosyl-l-methionine (SAM) superfamily of enzymes, is an interferon-inducible protein implicated in the inhibition of replication of a broad range of RNA and DNA viruses, including dengue virus, West Nile virus, hepatitis C virus, influenza A virus, rabies virus and HIV. Viperin catalyses the conversion of cytidine triphosphate (CTP) to 3ʹ-deoxy-3′,4ʹ-didehydro-CTP (ddhCTP), a previously undescribed biologically relevant molecule, via a SAMdependent radical mechanism [1].

ddhCTP, can be produced in mammalian cytosol, is a chain terminator for the RNA-dependent RNA polymerases from multiple members of the Flavivirus genus, and directly inhibits replication of Zika virus in vivo[1].

 

Reference:

[1] Anthony S. Gizzi, Tyler L. Grove, Jamie J. Arnold, Joyce Jose, Rohit K. Jangra, Scott J. Garforth, Quan Du, Sean M. Cahill, Natalya G. Dulyaninova, James D. Love, Kartik Chandran, Anne R. Bresnick, Craig E. Cameron, Steven C. Almo. A naturally occurring antiviral ribonucleotide encoded by the human genome. Nature, 2018; DOI: 10.1038/s41586-018-0238-4.

Chemical Properties

Physical AppearanceSolution
StorageStore at -20°C
M.Wt465.14
FormulaC9H14N3O13P3
Synonyms3ʹ-deoxy-3′,4ʹ-didehydro-CTP
SolubilitySoluble in H2O
Chemical Name(5-(4-amino-2-oxopyrimidin-1(2H)-yl)-4-hydroxy-4,5-dihydrofuran-2-yl)methyl tetrahydrogen triphosphate
SDFDownload SDF
Canonical SMILESOC1C=C(COP(OP(OP(O)(O)=O)(O)=O)(O)=O)OC1N2C(N=C(N)C=C2)=O
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while.

Protocol

Cell experiment [1]:

Cell lines

Mammalian HEK293T cells.

Preparation method

This compound can be soluble in H2O. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Reacting condition

0~72 h, 0, 1, 10, 100 and 300 μM

Applications

ddhCTP inhibits replication of flavivirus (dengue virus, West Nile Virus and Zika virus) RNA-dependent RNA polymerase in HEK293T cells.

References:

[1] Anthony S. Gizzi, Tyler L. Grove, Jamie J. Arnold, Joyce Jose, Rohit K. Jangra, Scott J. Garforth, Quan Du, Sean M. Cahill, Natalya G. Dulyaninova, James D. Love, Kartik Chandran, Anne R. Bresnick, Craig E. Cameron, Steven C. Almo. A naturally occurring antiviral ribonucleotide encoded by the human genome. Nature, 2018; DOI: 10.1038/s41586-018-0238-4.

Quality Control

Quality Control & MSDS

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Chemical structure

ddhCTP

Related Biological Data

ddhCTP

Related Biological Data

ddhCTP