Home >> Cy3.5 azide (non-sulfonated)
Related Products
Cy3.5 azide (non-sulfonated)
used for Click chemistry to label alkyne-modified oligonucleotides

Catalog No.A8112
Size Price Stock Qty
5mg
$460.00
In stock
25mg
$1,350.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

      

Sample solution is provided at 25 µL, 10mM.

Quality Control

Chemical structure

Cy3.5 azide

Related Biological Data

Cy3.5 azide

Cy3.5 azide (non-sulfonated) Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

Cy3.5 azide (non-sulfonated) Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Chemical Properties

Cas No. SDF Download SDF
Chemical Name 3-(6-((3-azidopropyl)amino)-6-oxohexyl)-2-((1E,3E)-3-(3-butyl-1,1-dimethyl-1H-benzo[e]indol-2(3H)-ylidene)prop-1-en-1-yl)-1,1-dimethyl-1H-benzo[e]indol-3-ium
Canonical SMILES CC1(C)C2=C3C(C=CC=C3)=CC=C2[N+](CCCCCC(NCCCN=[N+]=[N-])=O)=C1/C=C/C=C4C(C)(C)C5=C(C=CC=C6)C6=CC=C5N\4CCCC
Formula C44H53ClN6O M.Wt 717.38
Solubility ≥71.7mg/mL in DMSO Storage 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Spectral properties

Excitation max (nm) 591 Emission max (nm) 604
Extinction Coefficient (M-1cm-1) 116000 Quantum Yield 0.35
CF260 0.29 CF280 0.22

Application

Non-sulfonated cyanines are available for the labeling of many targets including:

• Soluble proteins, which are tolerant to addition of organic co-solvent • Fluorescence polarization (FP)
• Antibodies (with 5-10% of DMSO/DMF) • Fluorescence resonance energy transfer (FRET)
• DNA and oligonucleotides • Time-resolved fluorescence energy transfer (TR-FRET)
• Peptides • Fluorescence intensity (FI)
• Many small molecules  
• Reactions in organic media (dichloromethane, acetonitrile)  

Background

Cy3.5 azide is a solid material dye ready for the use in Click Chemistry reaction. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

Cy3.5 azide could easily interfere with a radical polymerisation process as it contains a variety of double bonds. The Cy-3 modified initiator was then used to grow the modified PMPC-PDPA directly from the dye [1].

Reference:
[1] Gaitzsch, J. ; Delahaye, M.; Poma, A.; Du Prez, F.; Battaglia, G. Comparison of metal free polymer-dye conjugation strategies in protic solvents. Polymer Chemistry, 2016, 7(17), 3046–3055.