|Cy3.5 azide (non-sulfonated)
used for Click chemistry to label alkyne-modified oligonucleotides
Sample solution is provided at 25 µL, 10mM.
Publications citing ApexBio Products
|Cas No.||SDF||Download SDF|
|Solubility||≥71.7mg/mL in DMSO||Storage||24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Excitation max (nm)||591||Emission max (nm)||604|
|Extinction Coefficient (M-1cm-1)||116000||Quantum Yield||0.35|
Non-sulfonated cyanines are available for the labeling of many targets including:
|• Soluble proteins, which are tolerant to addition of organic co-solvent||• Fluorescence polarization (FP)|
|• Antibodies (with 5-10% of DMSO/DMF)||• Fluorescence resonance energy transfer (FRET)|
|• DNA and oligonucleotides||• Time-resolved fluorescence energy transfer (TR-FRET)|
|• Peptides||• Fluorescence intensity (FI)|
|• Many small molecules|
|• Reactions in organic media (dichloromethane, acetonitrile)|
Cy3.5 azide is a solid material dye ready for the use in Click Chemistry reaction. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
Cy3.5 azide could easily interfere with a radical polymerisation process as it contains a variety of double bonds. The Cy-3 modified initiator was then used to grow the modified PMPC-PDPA directly from the dye .
 Gaitzsch, J. ; Delahaye, M.; Poma, A.; Du Prez, F.; Battaglia, G. Comparison of metal free polymer-dye conjugation strategies in protic solvents. Polymer Chemistry, 2016, 7(17), 3046–3055.