|Chelerythrine Chloride PKC antagonist|
Sample solution is provided at 25 µL, 10mM.
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|Cas No.||34316-15-9||SDF||Download SDF|
|Solubility||≥19.2 mg/mL in DMSO, ≥9.45 mg/mL in EtOH with ultrasonic and warming, <2.66 mg/mL in H2O||Storage||Store at -20°C|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
Chelerythrine is a potent, selective antagonist of PKC (protein kinase C) with IC50 value of 0.66 μM.
The alkaloid chelerythrine is a highly specific inhibitor that acts at the regulatory domain of the kinase. It is also a competitive inhibitor with respect to the phosphate acceptor and a non-competitive inhibitor with respect to ATP. Chelerythrine induced a dose-dependent decrease in the cell viability with IC50 value of 2.6 μM measured by MTT reduction assay. Chelerythrine is also a selective and strong inhibitor of Bcl-xL functions and induced cell death in MEF cells with IC50 value of 1.1 μM. Chelerythrine activated MEKK1- and MKK4-dependent JNK1 and p38 pathways then mediated the induction of apoptosis. Chelerythrine stimulated apoptosis in the in vivo rat experiments (5 mg/kg) by inducing the generation of reactive oxygen species. Chelerythrine also has widespread physiological effects on primarily antimicrobial and anti-inflammatory.
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