Calcium Ionophore I
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Calcium Ionophore I, also known as CA 1001 or ETH 1001, is a Ca2+-selective ionophore .
Ionophores can selectively change the ionic permeability of a membrane and therefore can be used to mimic the properties of the ion carriers normally found in biological membranes and to study membrane function .
Calcium Ionophore I is a Ca2+-selective neutral carrier. In Ca2+ loaded IOVs derived from human RBCs, ETH 1001 promoted Ca2+ flux across RBC membranes. The amount of Ca2+ in cells treated with ETH 1001 was significantly higher than in control cells. In the presence of external Ca2+, ETH 1001 was capable of transporting Ca2+ into RBCs . CA 1001 was also used to construction of the Ca2+-selective membrane microelectrode .
Dagostino M, Lee CO. Neutral carrier Na+- and Ca2+-selective microelectrodes for intracellular application. Biophys J. 1982 Dec;40(3):199-207.
Caroni P, Gazzotti P, Vuilleumier P, et al. Ca2+ transport mediated by a synthetic neutral Ca2+ -ionophore in biological membranes. Biochim Biophys Acta. 1977 Nov 1;470(3):437-45.
Hinds TR, Vincenzi FF. The effect of ETH 1001 on ion fluxes across red blood cell membranes. Cell Calcium. 1985 Jun;6(3):265-79.
Lücke A, Lehmenkühler A, Speckmann EJ. The influence of the convulsant pentylenetetrazol on Ca2+-selective microelectrodes (neutral carrier ETH 1001). J Neurosci Methods. 1989 Aug;29(2):139-42.
Lee MH, Yoo CL, Lee JS, et al. Tweezer-type neutral carrier-based calcium-selective membrane electrode with drastically reduced anionic interference. Anal Chem. 2002 Jun 1;74(11):2603-7.
|Physical Appearance||A solution in ethanol|
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Chemical Name||(17R,18R)-diethyl 13,17,18,22-tetramethyl-14,21-dioxo-16,19-dioxa-13,22-diazatetratriacontane-1,34-dioate|
|Canonical SMILES||C[[email protected]]([[email protected]](OCC(N(CCCCCCCCCCCC(OCC)=O)C)=O)C)OCC(N(C)CCCCCCCCCCCC(OCC)=O)=O|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|