Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit
α-Ketoglutarate (α-KG) is a keto acid and is an important intermediate in the Krebs cycle, locating after isocitrate and before succinyl CoA. Anaplerotic reactions can replenish the Krebs cycle by synthesizing α-KG from glutamate through the action of glutamate dehydrogenase or from transamination of glutamate. α-KG is also an important nitrogen transporter. Measurement of α-KG in newborns is an indicator of inborn errors of metabolism.
The Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit provides a highly sensitive, fast and convenient way for detection of α-KG levels in various biological samples based on colorimetric and fluorometric method. In the assay, α-KG is transaminated with the production of pyruvate which is utilized to transform a nearly colorless probe to both fluorescence (Ex/Em = 535/587 nm) and color (λmax = 570 nm) product. The kit can detect α-KG in the range of 0.01 to 10 nmoles.