Toggle Nav
Close
  • Menu
  • Setting

Caspase-4 Fluorometric Assay Kit

Catalog No.
K2198
Convenient, Highly-Sensitive Assay.
Grouped product items
SizePriceStock Qty
25 assays
$174.00
In stock
100 assays
$414.00
In stock
200 assays
$594.00
In stock
400 assays
$918.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Caspase-4 belongs to the Caspase-family of cysteine proteases. Caspase-4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue. Caspase-4 exists in cells as an inactive proenzyme. It is matured by proteolysis. The active Caspase-4 is a heterotetramer contain two large and two small subunits. The function of Caspase 4 is believed to be an inflammatory caspase, together with Caspase-1, Caspase-5, with a role in the immune system.

Apoptosis in mammalian cells is caused by activation of ICE-family proteases/caspases. The Caspase-4 Fluorometric Assay Kit provides a fast and simple means to assay the activity of caspases that recognize the sequence LEVD. The assay is according to detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (400 nm); free AFC emits a yellow-green fluorescence (505 nm) upon cleavage of the substrate by Caspase-4 or other related Caspases. Using a fluorometer or a fluorecence microtiter plate reader can quantify the fluorescence. Comparison of the fluorescence of AFC from an apoptotic sample with an un-induced control allows determination of the fold increase in LEVD-dependent Caspase activity.

Features & Properties

FeaturesFast and convenient; Simple one-step procedure; Takes only 1-2 hours; Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity.
ShippingGel pack.
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

View current batch:
 

Overview

Kit componentsCell Lysis Buffer, 2X Reaction Buffer, LEVD-AFC (1 mM), DTT (1 M)