Caspase-4 Fluorometric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Caspase-4 belongs to the Caspase-family of cysteine proteases. Caspase-4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue. Caspase-4 exists in cells as an inactive proenzyme. It is matured by proteolysis. The active Caspase-4 is a heterotetramer contain two large and two small subunits. The function of Caspase 4 is believed to be an inflammatory caspase, together with Caspase-1, Caspase-5, with a role in the immune system.
Apoptosis in mammalian cells is caused by activation of ICE-family proteases/caspases. The Caspase-4 Fluorometric Assay Kit provides a fast and simple means to assay the activity of caspases that recognize the sequence LEVD. The assay is according to detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (400 nm); free AFC emits a yellow-green fluorescence (505 nm) upon cleavage of the substrate by Caspase-4 or other related Caspases. Using a fluorometer or a fluorecence microtiter plate reader can quantify the fluorescence. Comparison of the fluorescence of AFC from an apoptotic sample with an un-induced control allows determination of the fold increase in LEVD-dependent Caspase activity.
Features & Properties
|Features||Fast and convenient; Simple one-step procedure; Takes only 1-2 hours; Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
- View current batch:
|Kit components||Cell Lysis Buffer, 2X Reaction Buffer, LEVD-AFC (1 mM), DTT (1 M)|