Caspase-10 Colorimetric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Apoptosis in mammalian cells is caused by activation of ICE-family proteases/caspases. The Caspase-10 Colorimetric Assay Kit provides a fast and simple means for assaying the activity of caspases that recognize the sequence AEVD. The assay is according to spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate AEVD-pNA. Using a spectrophotometer or a microtiter plate reader at 400 or 405 nm can quantify the pNA light emission. Comparison of the absorbance of pNA from an apoptotic sample with an un-induced control allows determination of the fold increase in AEVD-dependent caspase activity.
Features & Properties
|Features||Fast and convenient; Simple one-step procedure; Takes only 1-2 hours; Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in AEVD-dependent Caspase activity.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
- View current batch:
|Kit components||Cell Lysis Buffer, 2X Reaction Buffer, AEVD-pNA (4 mM), DTT (1 M), Dilution Buffer|