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Caspase-10 Colorimetric Assay Kit

Catalog No.
K2197
Convenient, highly-sensitive assay.
Grouped product items
SizePriceStock Qty
25 assays
$174.00
In stock
100 assays
$414.00
In stock
200 assays
$594.00
In stock
400 assays
$918.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Apoptosis in mammalian cells is caused by activation of ICE-family proteases/caspases. The Caspase-10 Colorimetric Assay Kit provides a fast and simple means for assaying the activity of caspases that recognize the sequence AEVD. The assay is according to spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate AEVD-pNA. Using a spectrophotometer or a microtiter plate reader at 400 or 405 nm can quantify the pNA light emission. Comparison of the absorbance of pNA from an apoptotic sample with an un-induced control allows determination of the fold increase in AEVD-dependent caspase activity.

Features & Properties

FeaturesFast and convenient; Simple one-step procedure; Takes only 1-2 hours; Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in AEVD-dependent Caspase activity.
ShippingGel pack.
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

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Overview

Kit componentsCell Lysis Buffer, 2X Reaction Buffer, AEVD-pNA (4 mM), DTT (1 M), Dilution Buffer