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Caspase-5 Colorimetric Assay Kit

Catalog No.
K2196
Convenient, Highly-Sensitive Assay.
Grouped product items
SizePriceStock Qty
25 assays
$174.00
In stock
100 assays
$414.00
In stock
200 assays
$582.00
In stock
400 assays
$954.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Caspase-5 belongs to the Caspase-family of cysteine proteases. Caspase-5 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue. Caspase-5 exists in cells as an inactive proenzyme and is matured by proteolysis. The active Caspase-5 is a heterotetramer containing two large and two small subunits. The function of Caspase-5 is believed to be an inflammatory Caspase, together with Caspase-1, Caspase-4, and has a role in the immune system.

The Caspase-5 Colorimetric Assay Kit provides a fast and convenient means for assaying the activity of Caspase-5 and other related caspases. These caspases can recognize the sequence WEHD. The assay is according to spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate WEHD-pNA. Using a spectrophotometer or a microtiter plate reader at 400 or 405 nm can quantify the pNA light emission. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-5 activity.

Features & Properties

FeaturesFast and convenient; Simple one-step procedure; Takes only 1-2 hours; Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-5 activity.
ShippingGel pack.
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

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Overview

Kit componentsCell Lysis Buffer, 2X Reaction Buffer, WEHD-pNA (4 mM), DTT (1 M), Dilution Buffer