Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Alanine aminotransferase (ALT), also known as serum glutamic pyruvic transaminase (SGPT) or alanine transaminase (ALAT) is a transaminase enzyme. ALT can be found in serum and different body tissues and is mainly found in liver. It catalyzes the reaction: α-ketoglutarate + alanine ⇌ glutamate + pyruvate. It is commonly used as a clinical test for liver health. In Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate and produce pyruvate and glutamate. The pyruvate can be detected in a reaction that concomitantly transfrom a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). This kit offers a reliable and sensitive way for high throughput activity assay of ALT with a detection limit of 0.05 mU per well.
Features & Properties
|Features||Simple procedure; Fast and convenient Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
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|Kit components||ALT Assay Buffer OxiRed Probe (in DMSO) ALT Enzyme Mix ALT Substrate Pyruvate Standard (100 nmol/µl) ALT positive control|