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Caspase-5 Fluorometric Assay Kit

Catalog No.
K2195
Convenient, Highly-Sensitive Assay.
Grouped product items
SizePriceStock Qty
25 assays
$162.00
In stock
100 assays
$390.00
In stock
200 assays
$558.00
In stock
400 assays
$918.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Caspase-5 belongs to the Caspase-family of cysteine proteases. Caspase-5 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue. Caspase-5 exists in cells as an inactive proenzyme and is matured by proteolysis. The active Caspase-5 is a heterotetramer containing two large and two small subunits. The function of Caspase-5 is believed to be an inflammatory Caspase, together with Caspase-1, Caspase-4, and has a role in the immune system.

Apoptosis in mammalian cells is caused by activation of ICE-family proteases/caspases. The Caspase-5 Fluorometric Assay Kit provides a fast and convenient means to assay the activity of Caspase-5 and other related caspases. These caspases can recognize the sequence WEHD. The assay is according to the detection of cleavage of substrate WEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). WEHD-AFC emits blue light (λmax = 400 nm); free AFC emits a yellow-green fluorescence ( λmax = 505 nm) upon cleavage of the substrate by Caspase-5 or other related caspases. Using a fluorometer or fluorecence microtiter plate reader can quantify the fluorescence. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in Caspase-5 activity.

Features & Properties

FeaturesFast and convenient; Simple one-step procedure; Takes only 1-2 hours; Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.
ShippingGel pack.
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

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Overview

Kit componentsCell Lysis Buffer, 2X Reaction Buffer, WEHD-AFC (1 mM), DTT (1 M)