Pyruvate Colorimetric/Fluorometric Assay Kit
Pyruvate is an important intermediate in several metabolic pathways. Pyruvate is produced from glucose via glycolysis. Pyruvate can be converted to fatty acids via acetyl CoA or to carbohydrates via gluconeogenesis. Pyruvate is also used to stimulate metabolism which then leads to loss of body weight. High levels of pyruvate are associated with genetic disorders and liver disease.
The Pyruvate Colorimetric/Fluorometric Assay Kit provides a simple and convenient way for detection of pyruvate in various biological samples such as culture and fermentation media, blood and cells based on colorimetric and fluorometric method. In the assay, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to yield fluorescence (at Ex/Em = 535/587 nm) and color (at λ= 570 nm). The fluorescence or color intensity is proportional to pyruvate content. The assay provides a direct and automation-ready procedure for accurately measuring pyruvate concentration. The kit can detect 1 µM to 10 mM pyruvate.