NAD/NADH Quantitation Colorimetric Kit
Nicotinamide adenine dinucleotide (NAD) is a coenzyme present in all living cells and exists in two forms: NAD+ and NADH. NAD plays important roles in energy transforming and redox state of cells or tissues.
The NAD/NADH Quantitation Colorimetric Kit provides a sensitive and convenient way for detection of the intracellular nucleotides: NADH, NAD and their ratio. The NAD Cycling Enzyme Mix specifically recognizes NADH/NAD in an enzyme cycling reaction, which are not required to purify from samples. The enzyme cycling reaction significantly increases the detection specificity and sensitivity. By comparing with standard NADH, NADH or NADt (NAD and NADH) can be easily quantified. The assay specifically detects NAD and NADH, but not NADPH or NADP.