SCF, human recombinant protein
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor at the early stages of hematopoiesis. SCF promotes the survival, differentiation, and mobilization of myeloid, erythroid, megakaryocytic, lymphoid, germ cell and melanocyte progenitors. SCF exists in two forms: cell surface bound SCF and soluble SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.
The human SCF cDNA encodes a 273 aa protein contains a 25 aa signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. Human SCF shows low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant human SCF is a 18.4 kDa protein containing 165 amino acid residues.
| Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. | ||||||
| ED50 | < 2 ng/ml. | |||||
| Specific activity | > 5*105 units/mg. | |||||
Quality Control & DataSheet
- View current batch:
-
Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
Related Biological Data
| Gene ID: | 4254 | Accession #: | P21583 |
| Alternate Names: | Human SCF, SCF, h-SCF, rh-SCF, recombinant human SCF, recombinant SCF. | ||
| Source: | Escherichia Coli. | M.Wt: | 18.9 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20°C |
| Formulation: | Lyophilized from 10 mM Acetic Acid. | ||
| Solubility: | Reconstitute in sterile water to a concentration of 0.1-1 μg/μl. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use. | ||
| Shipping Condition: | Gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||