IL-21, human recombinant protein
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Interleukin-21 (IL-21) is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. IL-21 induces the differentiation of T-cell-stimulated B-cells into plasma cells and memory B-cells, apoptotic effects in naïve B-cells and stimulated B-cells in the absence of T-cell signaling and stimulates IgG production in conjunction with IL-4. Also, IL-21 enhances the anti-tumor activity of CD8+ T-cells and NK cells. The IL21/IL21 R interaction plays important roles in B and T-cell proliferation after antigen stimulation and NK cell maturation.
The human IL-21 cDNA encodes a 155 aa protein with a 22 aa signal sequence. Recombinant human IL-21 is a homodimeric, non-glycosylated polypeptide containing 133 amino acid residues and has a molecular weight of 15.5 kDa.
|Determined by the dose-dependent stimulation of human peripheral blood mononuclear cells.|
|ED50||< 5 ng/ml.|
Quality Control & DataSheet
- View current batch:
Purity: ≥90%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
Related Biological Data
Related Biological Data
|Gene ID:||59067||Accession #:||Q9HBE4|
|Alternate Names:||Human IL-21, IL-21, IL21, IL 21, h-IL-21, rh-IL-21, recombinant human IL-21, recombinant IL-21, IL, interluekin.|
|Source:||Escherichia Coli.||M.Wt:||15.5 kDa|
|Appearance:||Lyophilized protein||Storage:||Store at -20°C|
|Formulation:||Lyophilized (from 1 mg/ml solution containing no additives).|
|Solubility:||Reconstitute in H2O to a concentration of 0.1-1.0 µg/µl (Note: Gently pippetting to dissolve). The solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use.|
|Shipping Condition:||Gel pack.||Handling:||Centrifuge the vial prior to opening.|
|Usage:||For Research Use Only! Not to be used in humans.|