TPO, human recombinant protein
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Thrombopoietin (TPO), also known as megakaryocyte growth and development factor (MGDF), is a membrane-bound glycoprotein produced in the liver, kidney and skeletal muscle. TPO plays important roles in megakaryocytopoiesis and thrombopoiesis. TPO stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor.
The human TPO gene encodes a 353 aa protein contains a 21 aa signal sequence, a 15 amino acid erythropoietin-like domain, and a highly glycosylated 179 amino acid C-terminal domain. Recombinant human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein.
|Determined by the dose-dependent stimulation of the proliferation of human MO7e cells.|
|ED50||< 1 ng/ml.|
|Specific activity||> 1*106 units/mg.|
Quality Control & DataSheet
- View current batch:
Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
Related Biological Data
|Gene ID:||7173||Accession #:||P07202|
|Alternate Names:||Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF.|
|Source:||Escherichia Coli.||M.Wt:||18.6 kDa|
|Appearance:||Lyophilized protein||Storage:||Store at -20°C|
|Formulation:||Lyophilized with no additives.|
|Solubility:||Reconstitution in purified water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).|
|Shipping Condition:||Gel pack.||Handling:||Centrifuge the vial prior to opening.|
|Usage:||For Research Use Only! Not to be used in humans.|