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Nigericin sodium salt

In stock
Catalog No.
B7644
ionophore that exchanges K+ for H+ across biological membranes
Grouped product items
SizePriceStock Qty
5mg
$45.00
In stock
10mg
$70.00
In stock
25mg
$140.00
In stock

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Nigericin sodium salt is an ionophore that exchanges K+ for H+ across biological membranes.

Ionophore is a lipid-soluble molecule that transports ions across the cell membrane.

In medium with Na+, nigericin scarcely affected ADP-induced platelets aggregation, and slightly inhibited thrombin-induced platelets aggregation. Also, nigericin decreased the cytoplasmic pH in the medium with Na+. The effects of nigericin on platelet aggregation are mainly due to its effects on the cytoplasmic pH [1]. The K+ ionophore nigericin is highly effective as an ionophore for Pb2+. Physiological concentrations of Ca2+ or Mg2+ didn’t inhibit nigericin-catalyzed Pb2+ transport, while K+ and Na+ concentrations (0-100 mM) modestly affected Pb2+ transport. Nigericin may be helpful in the treatment of Pb intoxication [2]. At two concentrations representing the low (5uM) and high ATP (1.5mM) ranges, Nigericin inhibited the ATP-driven transhydrogenase reaction at both ranges with a more pronounced effect on the low ATP concentration. At a concentration of 2.5 mM ATP, nigericin tripled the Oxonol response [3].

Reference:
[1].  Kitagawa S, Awai M, Kametani F. Relationship of the effects of nigericin on the aggregation and cytoplasmic pH of bovine platelets in the presence of different cations. Biochim Biophys Acta, 1987, 930(1): 48-54.
[2].  Hamidinia SA, Tan B, Erdahl WL, et al. The ionophore nigericin transports Pb2+ with high activity and selectivity: a comparison to monensin and ionomycin. Biochemistry, 2004, 43(50): 15956-15965.
[3].  Eytan GD, Carlenor E, Rydstr m J. Energy-linked transhydrogenase. Effects of valinomycin and nigericin on the ATP-driven transhydrogenase reaction catalyzed by reconstituted transhydrogenase-ATPase vesicles. J Biol Chem, 1990, 265(22): 12949-12954.

Product Citation

Chemical Properties

StorageStore at -20°C
M.Wt746.94
Cas No.28643-80-3
FormulaC40H67NaO11
Solubility≥74.7mg/mL in EtOH, <3.735mg/mL in DMSO
Chemical Namesodium (S)-2-((2R,3S,6R)-6-(((2S,4S,5R,7R,9S,10R)-2-((2R,2'R,3'S,5R,5'R)-5'-((2S,3S,5R,6R)-6-hydroxy-6-(hydroxymethyl)-3,5-dimethyltetrahydro-2H-pyran-2-yl)-2,3'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-9-methoxy-2,4,10-trimethyl-1,6-dioxaspiro[4.5]decan-7-
SDFDownload SDF
Canonical SMILESO[[email protected]@]1(CO)O[[email protected]@H]([[email protected]@H](C)C[[email protected]]1C)[[email protected]@H]2O[[email protected]]([[email protected]@H](C)C2)[[email protected]]3(C)O[[email protected]](CC3)[[email protected]@]4(C)O[[email protected]]([[email protected]@H](C)C4)([[email protected]@H]5C)O[[email protected]](C[[email protected]@H]6O[[email protected]@H]([[email protected]@H](C([O-])=O)C)[[email protected]@H](C)CC6)C[[email protected]@H]5OC.[Na+]
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Protocol

Cell Experiment [1]:

Clone

Blood Latelets

Preparation Method

Solubility <3.7mg/ml in DMSO. If a higher concentration of solution, the general steps are as follows: please send a test tube heating at 37 ° C for 10 minutes and/or place it in the ultrasonic bath in shock for a period of time. Concentrate on - 20 ° C can be placed for months.

Reaction Condition

2 μM;2minutes

Experimental Result

In medium of K + (combined with ion carrier, with the highest permeability), Nigericin Sodium Salt enhanced platelet aggregation. However, in the medium of choline (combined with ion carrier, has relatively low permeability), Nigericin Sodium Salt inhibited platelet aggregation. In addition, relative to the platelet aggregation rate, Nigericin Sodium Salt have a greater influence on the maximum of platelet aggregation.

References:

[1]. Kitagawa S, Awai M, Kametani F. Relationship of the effects of nigericin on the aggregation and cytoplasmic pH of bovine platelets in the presence of different cations. Biochim Biophys Acta, 1987, 930(1): 48-54.

Quality Control

Chemical structure

Nigericin sodium salt

Related Biological Data

Nigericin sodium salt

Related Biological Data

Nigericin sodium salt

Related Biological Data

Nigericin sodium salt