In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
DL-threo-2-methylisocitrate sodium is a substrate of isocitratelyase 1(ICL1).
ICL1 had dual activity for both threo-D(s)L(s)-isocitrate (ICA) and DL-threo-2-methylisocitrate (MICA). Using Michaelis-Menten non-linear least squares fit, the Km of purified recombinant ICL1 for threo-D(s)L(s)-isocitrate (ICA) was determined to be 188 mM with akcatof 5.24 s-1 .The Km of ICL2 for MICA was greater than 15 mM which was too high to be determained accurately due to the limitations of the solubility of MICA. The Kcat/Km for ICL1, a measureforcatalyticefficiency, was 2.79 × 104 M-1 s-1 and 1.74 ×103 M-1 s-1, for ICA and MICA respectively.
. Gould TA1,van de Langemheen H,Muoz-Elías EJ,McKinney JD,Sacchettini JC. Dual role of isocitratelyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis.Mol Microbiol.2006 Aug;61(4):940-7.
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Chemical Name||sodium (2R,3S)-3-hydroxybutane-1,2,3-tricarboxylate|
|Canonical SMILES||C[[email protected]](C([O-])=O)(O)[[email protected]@](C([O-])=O)([H])CC([O-])=O.C[[email protected]](C([O-])=O)(O)[[email protected]@](C([O-])=O)([H])CC([O-])=O.[Na+].[Na+].[Na+].[Na+].[Na+].[Na+]|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|