iMAC2 is the inhibitor of mitochondrial apoptosis-induced channel (MAC) with the IC50 value of 28 nM. MAC is a putative cytochrome c release channel involved in the intrinsic apoptotic pathway. MAC has been associated with oligomeric, not monomeric, Bax. By blocking MAC and suppressing cytochrome c release, iMAC2 suppressed the onset of apoptotic markers .
In vitro: In cultured apoptotic FL5.12 cells, pre-treatment with iMAC2 resulted in a normal volume and residual Annexin V/PI staining. iMAC2 reduced apoptosis by more than 50%. Pre-treatment with iMAC2 induced a significant protection at low micromolar levels (IC50 = 2.5 μM). Pre-incubation with iMAC2 and iMAC3 essentially eliminated the release of cytochrome c (P < 0.0001) while nanomolar levels of iMAC2 completely blocked the current flow through MAC. iMAC2 inhibited release of cytochrome c by Bid-induced Bax activation with the IC50 values of 0.68 μM .
 Peixoto PM1, Ryu SY, Bombrun A, Antonsson B, Kinnally KW. MAC inhibitors suppress mitochondrial apoptosis.Biochem J. 2009 Oct 12;423(3):381-7.
 Dejean L M, Martinez-Caballero S, Guo L, et al. Oligomeric Bax is a component of the putative cytochrome c release channel MAC, mitochondrial apoptosis-induced channel[J]. Molecular biology of the cell, 2005, 16(5): 2424-2432.
|Physical Appearance||White solid|
|Storage||Desiccate at RT|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|