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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BIBO 3304 is a high affinity NPY Y1 receptor antagonist with IC50 values of 0.72 and 0.38 nM at rat and human receptors respectively [1].Neuropeptide Y is a 36 amino acid polypeptide that is expressed in the hypothalamus. It is an endocrine and neuronal messenger involved in many physiological processes such as elevates blood pressure and stimulates food intake [1]. BIBO 3304 displayed high affinity (IC50=0.69+0.16 nM) for the human Y1 receptor stably expressed in BHK cells and a higher affinity(IC50=0.38+0.06 nM) for SK-N-MC cells, a human neuroblastoma cell line endogenously expressing the Y1 receptor. BIBO 3304 exhibited selective binding to the Y1 receptor subtype and more than 1000 ± 10,000-fold lower affinity for the human Y2 receptor, the human and rat Y4 receptor as well as the human and rat Y5 receptor. In SK-N-MC cells, the NPY induced inhibition of cAMP synthesis was antagonized by 100 nM BIBO 3304 with a pKb of 9.1+0.4 [1].In a rat model, BIBO 3304 inhibited feeding response mediated by 1mg NPY in a dose-dependent way. A dose of 30mg caused an approximately 50% inhibition (1.87+0.3 g, n=18). BIBO 3304 (30mg) had no effect on noradrenaline or galanin induced feeding. However, it can block the feeding response mediated by NPY (2 ± 36) (1mg), NPY (3 ± 36) (1mg) and [Leu31, Pro34]NPY (2mg) [1]. BIBO 3304 also antagonizes anxiolytic-like effects of NPY in the basolateral nucleus of the amygdala in rats [2].References:[1]. Wieland HA, Engel W, Eberlein W, et al. Subtype selectivity of the novel nonpeptide neuropeptide Y Y1 receptor antagonist BIBO 3304 and its effect on feeding in rodents. Br J Pharmacol, 1998, 125(3): 549-55.[2]. Sajdyk TJ, Vandergriff MG, Gehlert DR. Amygdalar neuropeptide Y Y1 receptors mediate the anxiolytic-like actions of neuropeptide Y in the social interaction test. Eur J Pharmacol, 1999, 5;368(2-3): 143-147.