In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Flavopiridol hydrochloride is a selective inhibitor of CDK1, CDK2, CDK4 and CDK6 with IC50 value all ~41 nM as well as CDK7 with 300nM   .
CDKs are protein kinase families which involve in the process of regulating transcription, mRNA processing, cell differentiation and cell cycle. It has been shown that CDKs are abnormally expressed in a variety of cells .
Flavopiridol hydrochloride is a potent CDK inhibitor and is different from UCN-01. When tested with breast carcinoma cell line MCF-7, flavopiridol hydrochloride treatment showed high inhibitory ability to arrest cell cycle at G1 phase through inhibiting CDK2 and CDK4 . Treated seven lymphoma cell lines with flavopiridol hydrochloride at the concentration of 400 nM, it showed that flavopiridol hydrochloride induced cells apoptosis by inhibiting CDK4, CDK6, CDK7 or CDK9 which in turn inhibited Rb phosphorylation, anti-apoptotic preteins (Mcl-1 and XIAP) .
In mouse model with HL-60 subcutaneous xenograft, administration of flavopiridol hydrochloride (7.5 mg/kg) intravenously induced advanced stage animals complete regression with 91.67% and remained disease-free several months after one course of flavopiridol treatment .
It has also been reported that flavopiridol hydrochloride inhibited epidermal growth factoe receptor and protein kinase A with IC50 value of 21 and 122 μM, respectively .
. Carlson, B.A., et al., Flavopiridol induces G1 arrest with inhibition of cyclin-dependent kinase (CDK) 2 and CDK4 in human breast carcinoma cells. Cancer Res, 1996. 56(13): p. 2973-8.
. Losiewicz, M.D., et al., Potent inhibition of CDC2 kinase activity by the flavonoid L86-8275. Biochem Biophys Res Commun, 1994. 201(2): p. 589-95.
. Senderowicz, A.M., The cell cycle as a target for cancer therapy: basic and clinical findings with the small molecule inhibitors flavopiridol and UCN-01. Oncologist, 2002. 7 Suppl 3: p. 12-9.
. Ema, Y., et al., Investigation of the cytotoxic effect of flavopiridol in canine lymphoma cell lines. Vet Comp Oncol, 2015.
. Arguello, F., et al., Flavopiridol induces apoptosis of normal lymphoid cells, causes immunosuppression, and has potent antitumor activity In vivo against human leukemia and lymphoma xenografts. Blood, 1998. 91(7): p. 2482-90.
|Storage||Store at -20°C|
|Solubility||≥21.9mg/mL in DMSO|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|