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Caspase-5 Inhibitor Drug Screening Kit (Fluorometric)

Catalog No.
K2161
Screens caspase-5 inhibitor, includes caspase inhibitor.
Grouped product items
SizePriceStock Qty
100 assays
$474.00
In stock

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Background

Caspases (Cysteine-dependent aspartate-directed proteases) are a family of cysteine proteases that play important roles in apoptosis, inflammation and necrosis. Sequential activation of caspases plays an important role in cell apoptosis. Inhibition of caspases can delay apoptosis, indicating a potential role in drug screening efforts. Caspase 5 is an inflammatory caspase and plays an important role in the immune system, along with caspase 1 and caspase 4.

 The Caspase-5 Inhibitor Drug Screening Kit (Fluorometric) provides a simple, fast and convenient way for screening of caspase-5 inhibitors based on fluorometric method. The synthetic peptide substrate WEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin) emits blue light (λmax = 400 nm). While cleavage of WEHD-AFC by active caspase-5, free AFC emits a yellow-green fluorescence (λmax = 505 nm) that can be quantified by a fluorecence microtiter plate reader or a fluorometer. Inhibitors can be directly added to the reaction and the efficacy of inhibition of caspase-5 activity can be determined by comparison of the fluorescence intensity in samples without and with the testing inhibitors.

Features & Properties

FeaturesFast and convenient; Simple one-step procedure; Takes only 1-1.5 hours; Inhibition of caspase-5 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
ShippingGel pack.
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

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Overview

Kit components2X Reaction Buffer, Caspase Substrate WEHD-AFC (1 mM), DTT (1 M), Active Caspase-5 (Lyophilized), Caspase Inhibitor, Z-VAD-FMK (2 mM)