Home >> BAPTA-AM
Related Products
BAPTA-AM calcium chelator, selective and membrane permeable

Catalog No.B4758
Size Price Stock Qty
10mM (in 1mL DMSO)
$80.00
In stock
10mg
$67.00
In stock
50mg
$237.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

      

Sample solution is provided at 25 µL, 10mM.

Product Citations

1. Wang Y, Lu S, et al. "Sonic hedgehog induces GLT-1 degradation via PKC delta to suppress its transporter activities." Neuroscience. 2017 Oct 6;365:217-225. PMID:28993237

Quality Control

Chemical structure

BAPTA-AM

Related Biological Data

BAPTA-AM

Biological Activity

Description BAPTA-AM is a selective, membrane-permeable calcium chelator.
Targets            
IC50            

Protocol

Cell experiment [1, 2]:

Cell lines

Human leukemia cell lines HL-60 and U937, Bovine chromaffin cells

Preparation method

The solubility of this compound in DMSO is >16.3mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

10 μM, 50 μM; 6 h;

Applications

In human leukemia cell lines HL-60 and U937, BAPTA/AM (10 μM) exhibited classic apoptotic morphology, and BAPTA/AM (50 μM) induced atypical features (e.g., cell swelling, chromatin clumping).Superfusion with BAPTA-AM (50 μM) induced a rapid (< 60 s) and reversible block of both IKCa and IK (~50%). Preincubation with BAPTAAM (50 μM, 30 min) or cell loading with the non-permeable active form of BAPTA (10 mM in the pipette solution) permanently blocked IKCa. BAPTA-AM superfusion (50 μM) blocked IK (~53%) after BAPTA-loading or BAPTA-AM preincubation.

Animal experiment [3]:

Animal models

Swiss (RjOrl) mice, Male C57BL/6J mice

Dosage form

0–10 mg/kg, 30 min

Application

In Swiss (RjOrl) mice, pretreatment with BAPTA-AM (0–10 mg/kg, 30 min) prevented locomotor stimulation produced by ethanol without altering basal locomotion. BAPTA-AM reversed ethanol-induced hypnotic effects. Following a drinking-in-the-dark methodology, male C57BL/6J mice were offered 20% v/v ethanol, tap water, or 0.1% sweetened water. BAPTA-AM pretreatment (0–5 mg/kg) dose-dependently reduced ethanol consumption while leaving water and sweetened water intake unaffected.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Grant S, Freemerman AJ, Gregory PC, et al. Induction of apoptotic DNA fragmentation and c-jun downregulation in human myeloid leukemia cells by the permeant Ca2+ chelator BAPTA/AM. Oncol Res, 1995, 7(7-8): 381-392.

[2]. Urbano FJ, Buo W. BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells. Neuroreport, 1998, 9(15): 3403-3407.

[3] Balio P, Monferrer L, Pastor R, et al. Intracellular calcium chelation with BAPTA-AM modulates ethanol-induced behavioral effects in mice. Exp Neurol, 2012, 234(2): 446-453.

BAPTA-AM Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

BAPTA-AM Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Chemical Properties

Cas No. 126150-97-8 SDF Download SDF
Canonical SMILES O=C(CN(CC(OCOC(C)=O)=O)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)OCOC(C)=O
Formula C34H40N2O18 M.Wt 764.68
Solubility >16.3mg/mL in DMSO Storage Desiccate at -20°C
Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Background

BAPTA-AM is a selective calcium chelator [1].

Ca2+ is one of the most ubiquitous and versatile intracellular signaling molecules that control numerous cellular processes such as neurotransmitter release, contraction of all muscle cell types and fertilization [4].

BAPTA-AM is a selective and membrane permeable calcium chelator. In the human leukemia cell lines HL-60 and U937, BAPTA/AM (10 μM) induced internucleosomal DNA cleavage and classic apoptotic morphology. Also, BAPTA/AM increased Ca2+ in intracellular and downregulated c-jun [1]. In bovine chromaffin cells, APTA-AM (50 μM) rapidly and reversibly inhibited Ca2+-activated K+ (I(KCa)) and voltage-gated K+ (I(K)) by 50% [2]. In HEK 293 cells, BAPTA-AM inhibited hERG (Kv11.1), hKv1.3 and hKv1.5 channels with IC50 values of 1.3, 1.45 and 1.23 μM respectively in a concentration dependent way, which was dependent on channel opening [3].

In swiss mice, BAPTA-AM inhibited locomotor stimulation produced by ethanol and reversed ethanol-induced hypnotic effects. In male C57BL/6J mice, BAPTA-AM reduced ethanol consumption in a dose-dependent way [4].

References:
[1].  Grant S, Freemerman AJ, Gregory PC, et al. Induction of apoptotic DNA fragmentation and c-jun downregulation in human myeloid leukemia cells by the permeant Ca2+ chelator BAPTA/AM. Oncol Res, 1995, 7(7-8): 381-392.
[2].  Urbano FJ, Buño W. BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells. Neuroreport, 1998, 9(15): 3403-3407.
[3].  Tang Q, Jin MW, Xiang JZ, et al. The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells. Biochem Pharmacol, 2007, 74(11): 1596-1607.
[4].  Baliño P, Monferrer L, Pastor R, et al. Intracellular calcium chelation with BAPTA-AM modulates ethanol-induced behavioral effects in mice. Exp Neurol, 2012, 234(2): 446-453.