|Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit
Sensitive Assay, HTS
Sample solution is provided at 25 µL, 10mM.
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|Kit Components||Catalog No||K2171|
|AST Assay Buffer
AST Enzyme Mix
Pyruvate Standard (100 nmol/µl)
AST positive control
|Product name||Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit|
|Detection method||Absorbance (450 nm).|
|Sample type||Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.|
|Applications: This assay detects AST/ SGOT with a detection limit of 10 mU per well.|
Features & Properties
|Features||Simple procedure; Fast, reliable and convenient
Sensitive assays for measuring AST/ SGOT activity in various biological samples and the kit is also suitable for high throughput analyses.
|Storage Conditions||Store at -20°C.|
|Usage||For Research Use Only! Not For Use in Humans.|
Aspartate aminotransferase (AST), also known as Glutamate-oxaloacetate transaminase (GOT) is a transaminase that is related to the more liver specific alanine transaminase (ALT). It is not only commonly used in liver function test but also for study in various diseases (e.g. myocardial infarction, acute pancreatitis and acute hemolytic anemia etc. It catalyzes the following reaction: Aspartate + ⍺-Ketoglutarate ⇌ Oxaloacetate + Glutamate. In Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit, an amino group is transferred from aspartate to ⍺-ketoglutarate and produce oxaloacetate and glutamate which can be detected converts a nearly colorless probe to color (λmax = 450 nm). This kit offers a reliable and sensitive way for high throughput activity assay of AST with a detection limit of 10 mU per well.