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Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit
Sensitive Assay, HTS

Catalog No.K2171
Size Price Stock Qty
100 assays
$402.00
In stock

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Sample solution is provided at 25 µL, 10mM.

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Overview

Kit ComponentsCatalog NoK2171
AST Assay Buffer
AST Enzyme Mix
Developer
ALT Substrate
Pyruvate Standard (100 nmol/µl)
AST positive control
Product nameAspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit
Detection methodAbsorbance (450 nm).
Sample typeCell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.
Species reactivityMammalian
Applications: This assay detects AST/ SGOT with a detection limit of 10 mU per well.

Features & Properties

FeaturesSimple procedure; Fast, reliable and convenient
Sensitive assays for measuring AST/ SGOT activity in various biological samples and the kit is also suitable for high throughput analyses.
ShippingGel pack
Storage ConditionsStore at -20°C.
UsageFor Research Use Only! Not For Use in Humans.

Description

Aspartate aminotransferase (AST), also known as Glutamate-oxaloacetate transaminase (GOT) is a transaminase that is related to the more liver specific alanine transaminase (ALT). It is not only commonly used in liver function test but also for study in various diseases (e.g. myocardial infarction, acute pancreatitis and acute hemolytic anemia etc. It catalyzes the following reaction:  Aspartate + ⍺-Ketoglutarate   ⇌   Oxaloacetate + Glutamate. In Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit, an amino group is transferred from aspartate to ⍺-ketoglutarate and produce oxaloacetate and glutamate which can be detected converts a nearly colorless probe to color (λmax = 450 nm). This kit offers a reliable and sensitive way for high throughput activity assay of AST with a detection limit of 10 mU per well.