In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Apocynin is a selective NADPH-oxidase inhibitor with IC50 value of 10 μM .
Apocynin is an intracellular inhibitor of the assembly of NADPH oxidase, concomitantly inhibiting reactive oxygen species (ROS) production, which contributes to various anti-inflammatory effects [1, 2].
In human neutrophils stimulated by serum-treated zymosan (STZ), Apocynin completely inhibited oxygen uptake at the concentration of 300 μM. Inhibition of the STZ-induced respiratory burst by Apocynin was also observed in human eosinophils, but not in human alveolar macrophages .
In a mouse model of carrageenan-induced pleurisy, intraperitoneal injection of Apocynin at the dose of 5 mg/kg reduced lung injury and neutrophil infiltration. Apocynin also significantly reduced NADPH oxidase activity in lung tissues . In a mouse model of spinal cord injury, treatment with Apocynin (5 mg/kg, i.p.) 1 and 6 h after the trauma significantly decreased the degree of spinal cord inflammation and tissue injury. Moreover, Apocynin substantially ameliorated the loss of limb function .
. Stefanska J, Pawliczak R. Apocynin: molecular aptitudes. Mediators of Inflammation, 2008, 2008: 106507.
. Stolk J, Hiltermann T J, Dijkman J H, et al. Characteristics of the inhibition of NADPH oxidase activation in neutrophils by apocynin, a methoxy-substituted catechol. American Journal of Respiratory Cell and Molecular Biology, 1994, 11(1): 95-102.
. Impellizzeri D, Esposito E, Mazzon E, et al. Effect of apocynin, a NADPH oxidase inhibitor, on acute lung inflammation. Biochemical Pharmacology, 2011, 81(5): 636-648.
. Impellizzeri D, Mazzon E, Esposito E, et al. Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury. Free Radical Research, 2011, 45(2): 221-236.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥8.25mg/mL in DMSO|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37°C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.|