EZLink Sulfo-NHS-Biotin Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Components and Storage
|Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody|
|Streptavidin||10 mg||EZ-Link Sulfo-NHS-Biotin||25 mg|
|HABA Solution||1 mL||PBS Pack (makes 500 mL)||1 pack|
|Sephadex G-25 in PD-10 Desalting Columns||8.3 mL, 10 columns|
|Store biotin and streptavidin reagents at -20°C. Store remaining kit components at 4°C.|
|Label or Dye:||Biotin||Product Size:||10 reactions|
|Labeling Scale:||1-10 mg||Labeling Target:||Proteins (General), Antibodies (General)|
• Amine-reaction—Reacting with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides.
• Labeling antibody—This kit can label antibodies to facilitate immobilization, purification or detection.
• Labeling protein—This kit can label proteins to facilitate immobilization, purification or detection.
• Labeling Cell surface molecule—This kit can label the cell surface proteins because the negatively charged reagent does not permeate cell membranes.
• Irreversible-Permanent amide bonds are formed; Spacer arm cannot be cleaved.
• Very short arm—Spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only.
• Solubility increased—Sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds.
Sulfo-NHS-Biotin (N-Hydroxysulfosuccinimidobiotin) is an amine-reactive biotinylation reagent. The most common amine-reactive biotinylation reagents can be divied into two basic types, N-hydroxysuccinimide (NHS) esters and carboxylates. Generally, NHS esters biotinylation reagent contains a reactive NHS ring structure that reacts with an amine on the carbonyl group of a protein or other molecules through nucleophilic attack subsequently forming a stable amide linkage and releasing the NHS group. Due to the insolubility of NHS-biotin in aqueous environments, sulfo-NHS-biotin, a water-soluble analog of NHS-biotin, has been developed by attaching a negatively charged sulfonate group on the NHS ring structure, which can be added directly to aqueous reactions without the need for organic solvent dissolution.