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EZLink Sulfo-NHS-Biotin Kit

Catalog No.
Amine-reactive biotinyltation kit, water-soluble
Grouped product items
SizePriceStock Qty
10 reactions
In stock

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The EZLink Sulfo-NHS-Biotin Kit contains reagents sufficient for 10 biotin labeling reactions. It is a water-soluble biotin reagent for labeling protein, antibody or other molecules with amidogen (NH2-). The biotin in the kit is sufficient and has been activated for direct use. The whole procedure is simple and quick.

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Quality Control & DataSheet

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Components and Storage

Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
Streptavidin10 mgEZ-Link Sulfo-NHS-Biotin25 mg
HABA Solution1 mLPBS Pack (makes 500 mL)1 pack
Sephadex G-25 in PD-10 Desalting Columns8.3 mL, 10 columns
Store biotin and streptavidin reagents at -20°C. Store remaining kit components at 4°C.


Label or Dye:BiotinProduct Size:10 reactions
Labeling Scale:1-10 mgLabeling Target:Proteins (General), Antibodies (General)


• Amine-reaction—Reacting with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides.
• Labeling antibody—This kit can label antibodies to facilitate immobilization, purification or detection.
• Labeling protein—This kit can label proteins to facilitate immobilization, purification or detection.
• Labeling Cell surface molecule—This kit can label the cell surface proteins because the negatively charged reagent does not permeate cell membranes.
• Irreversible-Permanent amide bonds are formed; Spacer arm cannot be cleaved.
• Very short arm—Spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only.
• Solubility increased—Sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds.

Sulfo-NHS-Biotin (N-Hydroxysulfosuccinimidobiotin) is an amine-reactive biotinylation reagent. The most common amine-reactive biotinylation reagents can be divied into two basic types, N-hydroxysuccinimide (NHS) esters and carboxylates. Generally, NHS esters biotinylation reagent contains a reactive NHS ring structure that reacts with an amine on the carbonyl group of a protein or other molecules through nucleophilic attack subsequently forming a stable amide linkage and releasing the NHS group. Due to the insolubility of NHS-biotin in aqueous environments, sulfo-NHS-biotin, a water-soluble analog of NHS-biotin, has been developed by attaching a negatively charged sulfonate group on the NHS ring structure, which can be added directly to aqueous reactions without the need for organic solvent dissolution.