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EZLink NHS-LC-Biotin Kit

Catalog No.
Amine-reactive biotinyltation kit
Grouped product items
SizePriceStock Qty
10 reactions
In stock

Tel: +1-832-696-8203

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The EZLink NHS-LC-Biotin Kit contains reagents sufficient for 10 biotin labeling reactions. It is a water-soluble biotin reagent for labeling protein, antibody or other molecules with amidogen (NH2-). The biotin in the kit is sufficient and has been activated for direct use. The whole procedure is simple and quick.

Components and Storage

Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
Streptavidin10 mgEZ-Link NHS-LC-Biotin25 mg
HABA Solution1 mLPBS Pack (makes 500 mL)1 pack
Sephadex G-25 in PD-10 Desalting Columns8.3 mL, 10 columns
Store biotin and streptavidin reagents at -20°C. Store remaining kit components at 4°C.


Label or Dye:BiotinProduct Size:10 reactions
Labeling Scale:1-10 mgLabeling Target:Proteins (General), Antibodies (General)


• Amine-reaction—Reacting with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides.
• Labeling antibody—This kit can label antibodies to facilitate immobilization, purification or detection.
• Labeling protein—This kit can label proteins to facilitate immobilization, purification or detection.
• Labeling Cell surface molecule—This kit can label the cell surface proteins because the negatively charged reagent does not permeate cell membranes.
• Irreversible-Permanent amide bonds are formed; Spacer arm cannot be cleaved.
• Excellent balance—Spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass.
• Solubility increased—Sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds.

NHS-LC-biotin (succinimidyl-6-(biotinamindo)hexanoate), also known as NHS-X-biotin is a derivative of D-biotin, a amine-reactive biotinylation agent, that contains a spacer arm off the valeric acid side chain of D-biotin with an NHS ester group at its end. The NHS ester group at the end of NHS-LC-biotin covalently binds to amine groups in proteins and other molecules forming a stable amide linkage and releasing the NHS group. The 6-aminocaproic acid spacer of NHS-LC-biotin greatly increases the length between a covalently modified molecule and the bicyclic biotin rings leading to a better binding potential for avidin or streptavidin probes. NHS-LC-biotin is insoluble in aqueous environments requiring the dissolution of organic solvents prior to the addition to a buffered reaction.