|Amylase Activity Colorimetric Assay Kit
Detects amylase activity, sensitive.
Sample solution is provided at 25 µL, 10mM.
Quality Control & DataSheet
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Related Biological Data
|Kit Components||Catalog No||K2225|
|Amylase Assay Buffer
Amylase Substrate Mix
Amylase Positive Control
Nitrophenol standard (2 mM)
|Product name||Amylase Activity Colorimetric Assay Kit|
|Detection method||Absorbance (405 nm).|
|Sample type||Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples etc.|
|Applications: The assay can detect α-amylase content as low as 0.2 mU.|
Features & Properties
|Features||Fast and convenient.
Simple procedure; takes ~ less than 40 minutes.
Sensitive assays for measuring amylase activity in various biological samples.
|Storage Conditions||Store at -20℃.|
|Usage||For Research Use Only! Not For Use in Humans.|
Amylase is an enzyme that break starch down to sugar. Amylase is present in the saliva of humans. α-Amylase is the major form found in fungi, seeds, humans and other mammals. α-Amylase is a calcium metalloenzyme which completely unable to function in the absence of calcium. In humans, both the pancreatic and salivary amylases are major digestive enzymes. Increased amylase levels in humans are associated with salivary trauma, mumps because of renal failure, pancreatitis and inflammation of the salivary glands.
The Amylase Activity Colorimetric Assay Kit provides a sensitive, simple, fast and convenient way for detection of α-amylase activity in various samples based on colorimetric method. The assay utilizes ethylidene-pNP-G7 as the substrate. In the assay, the substrate is specifically cleaved by α-amylase to produce the smaller fragments, which can be acted upon by α-glucosidase that causes the ultimate release of the chromophore (at 405 nm). The kit can detect as low as 0.2 mU α-amylase content.