Amsacrine hydrochloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Amsacrine hydrochloride (Amsidine, m-AMSA) is an inhibitor of topoisomerase 2 [1].
Amsacrine hydrochloride is a chemotherapy drug of antineoplastic, used to treat some types of lymphoma and acute leukaemia. In addition, Amsacrine hydrochloride has shown the sensitivities to the cancer cell lines with the IC50 values of 190.2±27.4ng/ml, 46.1±3.9ng/ml,22.6±3.1ng/ml, 11.8±2.0ng/ml, 5.0±0.4ng/ml, and 11.7±1.5ng/ml for three bladder cancer cell lines (HT1376,RT112, RT4) and three testis cancer cell lines(833K, Susa, GH), respectively. And it has been found that the testis tumor cell lines are more sensitive to inhibiting by Amsacrine hydrochloride than the bladder cell lines [1].
References:
[1] Nelson EM, Tewey KM, Liu LF. Mechanism of antitumor drug action: poisoning of mammalian DNA topoisomerase II on DNA by 4'-(9-acridinylamino)-methanesulfon-m-anisidide. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1361-5.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 429.92 |
| Cas No. | 54301-15-4 |
| Formula | C21H20ClN3O3S |
| Solubility | ≥55.6mg/mL in DMSO |
| Chemical Name | N-[4-(acridin-9-ylamino)-3-methoxyphenyl]methanesulfonamide;hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | COC1=C(C=CC(=C1)NS(=O)(=O)C)NC2=C3C=CC=CC3=NC4=CC=CC=C42.Cl |
| Shipping Condition | Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months. |
Quality Control & MSDS
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Chemical structure
