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Amrubicin

In stock
Catalog No.
A8227
Topoisomerase II inhibitor, anthracycline agent
Grouped product items
SizePriceStock Qty
Evaluation Sample
$28.00
In stock
1mg
$150.00
In stock
5mg
$450.00
In stock

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Background

A synthetic anthracycline antibiotic. It inhibits DNA topoisomerase II. Antineoplastic.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt483.47
Cas No.110267-81-7
FormulaC25H25NO9
Solubility≥16.45mg/mL in DMSO, <2.37 mg/mL in EtOH, <2.16 mg/mL in H2O
Chemical Name(7S,9S)-9-acetyl-9-amino-7-[(2S,4S,5R)-4,5-dihydroxyoxan-2-yl]oxy-6,11-dihydroxy-8,10-dihydro-7H-tetracene-5,12-dione
SDFDownload SDF
Canonical SMILESCC(=O)C1(CC(C2=C(C3=C(C(=C2C1)O)C(=O)C4=CC=CC=C4C3=O)O)OC5CC(C(CO5)O)O)N
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Protocol

Cell experiment: [1]

Cell lines

CCRF-CEM cells

Preparation method

The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.

Reaction Conditions

IC 50: 3.3 µM, 1 hour

Applications

Amrubicin induced DNA-protein complex formation in cultured CCRF-CEM cells in a dose-dependent manner. The IC50 value of amrubicin required to inhibit the growth of CCRF-CEM cells was 3.3 µM. Accordingly, under conditions where cell growth was inhibited by amrubicin, considerable amounts of DNA-protein complexes were formed.

Animal experiment: [2]

Animal models

BALB/c nu/nu mice bearing Lu-24 or Lu-134 cells

Dosage form

Intravenous injection, 25 mg/kg

Applications

Amrubicin showed significant antitumor activities against both SCLC tumors tested, Lu-24 and Lu-134, with T/C-values at day 14 of 17% and 9%, respectively.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1] Hanada M, Mizuno S, Fukushima A, et al. A New Antitumor Agent Amrubicin Induces Cell Growth Inhibition by Stabilizing Topoisomerase II-DNA Complex. Cancer Science, 1998, 89(11): 1229-1238.

[2] Hanada M, Noguchi T, Yamaoka T. Amrubicin, a novel 9-aminoanthracycline, enhances the antitumor activity of chemotherapeutic agents against human cancer cells in vitro and in vivo. Cancer science, 2007, 98(3): 447-454.

Biological Activity

Description Amrubicin ia an inhibitor of Topoisomerase II Amrubicin.
Targets Topoisomerase II          
IC50            

Quality Control

Chemical structure

Amrubicin

Related Biological Data

Amrubicin

Related Biological Data

Amrubicin