|Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit
Sensitive Assay, HTS
Sample solution is provided at 25 µL, 10mM.
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|Kit Components||Catalog No||K2170|
|ALT Assay Buffer
OxiRed Probe (in DMSO)
ALT Enzyme Mix
Pyruvate Standard (100 nmol/µl)
ALT positive control
|Product name||Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit|
|Detection method||Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm).|
|Sample type||Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food etc.|
|Applications: The kit detects ALT with a detection limit of 10 mU per well.|
Features & Properties
|Features||Simple procedure; Fast and convenient
Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.
|Storage Conditions||Store at -20°C.|
|Usage||For Research Use Only! Not For Use in Humans.|
Alanine aminotransferase (ALT), also known as serum glutamic pyruvic transaminase (SGPT) or alanine transaminase (ALAT) is a transaminase enzyme. ALT can be found in serum and different body tissues and is mainly found in liver. It catalyzes the reaction: α-ketoglutarate + alanine ⇌ glutamate + pyruvate. It is commonly used as a clinical test for liver health. In Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate and produce pyruvate and glutamate. The pyruvate can be detected in a reaction that concomitantly transfrom a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). This kit offers a reliable and sensitive way for high throughput activity assay of ALT with a detection limit of 0.05 mU per well.