Ac-DEVD-CMK is a cell-permeable and irreversible caspase-3 inhibitor .
Apoptosis is a process of programmed cell death that occurs in multicellular organisms. Caspase are a family of protease enzymes playing essential roles in programmed cell death (including apoptosis, pyroptosis and necroptosis) and inflammation. Caspase activation is a major event in apoptosis. Caspase-3 cleaves and activates caspases 6 and 7, and is processed and activated by caspases 8, 9, and 10 .
Ac-DEVD-CMK (Ac-Asp-Glu-Val-Asp-CH2Cl) is a cell-permeable, irreversible and specific caspase-3 inhibitor. In coronary occlusion/reperfusion rat isolated hearts, Ac-DEVD-CMK reduced infarct size (the percentage of infarction 27.8+3.3% vs control 38.5+2.6%), suggesting that caspase inhibition during early reperfusion protected myocardium against lethal reperfusion injury . In BL41 cells, Ac-DEVD-CMK partially inhibited Mn2+-induced apoptosis and PARP cleavage, and partially blocked B cell death by 37% even at 100 μM . Ac-DEVD-CMK significantly blocked neurotoxicity at 24 hr after 1 hr of SIN-1 exposure and also protected against neurotoxicity at 24 hr after 90 min of zinc (75 μM) exposure. Ac-DEVD-CMK completely blocked SIN-1-induced activation of caspase-3 .
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|Storage||Store at -20°C|
|Synonyms||Ac-Asp-Glu-Val-Asp-CMK,Caspase-3 Inhibitor III|
|Solubility||≤50mg/ml in DMSO|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|