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4-Thio-UTP

In stock
Catalog No.
B7978
 
Grouped product items
SizePriceStock Qty
10ul (100 mM)
$172.00
Ship with 14 days
50ul (100 mM)
$824.00
Ship with 14 days
100ul (100 mM)
$1,512.00
Ship with 14 days

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Chemical Properties

StorageStore at -20°C or below
M.Wt500.2 g/mol (free acid)
FormulaC9H15N2O14P3S (free acid)
Synonyms4-Thiouridine-5'-Triphosphate, Thio UTP
Chemical Namelithium (3,4-dihydroxy-5-(2-oxo-4-thioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl triphosphate
SDFDownload SDF
Canonical SMILESS=C(C=CN1C2C(O)C(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O2)NC1=O.[Li+].[Li+].[Li+].[Li+]
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Protocol

In Vitro Experiments [1]
In vitro transcription assays and photoaffinity labelling of the transcriptional complex.

Methods

In vitro transcription reactions were carried out using RNA polymerase purified from lysed pea chloroplasts. The enzyme was incubated with the template DNA (5A/30T) containing the 16S rRNA promoter and a-32P-ATP (30 μCi, sp. act. 3000 Ci/mmole) and the photoprobe 4-thio UTP (200 μM) in the presence of 50mM Tris-HCl, pH 8.0, 10mM DTT, 1 μg BSA and 5mM MgCl2 at 25°C for 45 min.

Biological Activity

4-Thiouridine-5'-Triphosphate, also known as 4-Thio-UTP, is a potent agonist for P2Y2 and P2Y4 receptors. It is used in the study of crosslinking experiments, labeling of transcriptional complex. It also affects elongation and termination activity, causing transcriptional pause.

Quality Control

Quality Control & MSDS

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Chemical structure

4-Thio-UTP