In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
|Storage||Store at -20°C or below|
|M.Wt||500.2 g/mol (free acid)|
|Formula||C9H15N2O14P3S (free acid)|
|Synonyms||4-Thiouridine-5'-Triphosphate, Thio UTP|
|Chemical Name||lithium (3,4-dihydroxy-5-(2-oxo-4-thioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl triphosphate|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|In Vitro Experiments |
|In vitro transcription assays and photoaffinity labelling of the transcriptional complex.|
In vitro transcription reactions were carried out using RNA polymerase purified from lysed pea chloroplasts. The enzyme was incubated with the template DNA (5A/30T) containing the 16S rRNA promoter and a-32P-ATP (30 μCi, sp. act. 3000 Ci/mmole) and the photoprobe 4-thio UTP (200 μM) in the presence of 50mM Tris-HCl, pH 8.0, 10mM DTT, 1 μg BSA and 5mM MgCl2 at 25°C for 45 min.
|4-Thiouridine-5'-Triphosphate, also known as 4-Thio-UTP, is a potent agonist for P2Y2 and P2Y4 receptors. It is used in the study of crosslinking experiments, labeling of transcriptional complex. It also affects elongation and termination activity, causing transcriptional pause.|